Assuntos
Transtorno do Espectro Autista/terapia , Eletroconvulsoterapia/métodos , Comportamento Autodestrutivo/terapia , Adolescente , Agonistas de Receptores Adrenérgicos alfa 2/administração & dosagem , Transtorno do Espectro Autista/fisiopatologia , Clonidina/administração & dosagem , Humanos , Masculino , Reprodutibilidade dos Testes , Comportamento Autodestrutivo/fisiopatologia , Índice de Gravidade de Doença , Fatores de Tempo , Resultado do TratamentoAssuntos
Humanos , Masculino , Adolescente , Comportamento Autodestrutivo/terapia , Eletroconvulsoterapia/métodos , Transtorno do Espectro Autista/terapia , Fatores de Tempo , Índice de Gravidade de Doença , Reprodutibilidade dos Testes , Resultado do Tratamento , Comportamento Autodestrutivo/fisiopatologia , Clonidina/administração & dosagem , Agonistas de Receptores Adrenérgicos alfa 2/administração & dosagem , Transtorno do Espectro Autista/fisiopatologiaRESUMO
The studies of potentiation of 5-fluorouracil (5-FU), a traditional drug used in the treatment of several cancers, including colorectal (CRC), were carried out with zeolites Faujasite in the sodium form, with different particle sizes (NaY, 700nm and nanoNaY, 150nm) and Linde type L in the potassium form (LTL) with a particle size of 80nm. 5-FU was loaded into zeolites by liquid-phase adsorption. Characterization by spectroscopic techniques (FTIR, (1)H NMR and (13)C and (27)Al solid-state MAS NMR), chemical analysis, thermal analysis (TGA), nitrogen adsorption isotherms and scanning electron microscopy (SEM), demonstrated the successful loading of 5-FU into the zeolite hosts. In vitro drug release studies (PBS buffer pH 7.4, 37°C) revealed the release of 80-90% of 5-FU in the first 10min. To ascertain the drug release kinetics, the release profiles were fitted to zero-order, first-order, Higuchi, Hixson-Crowell, Korsmeyer-Peppas and Weibull kinetic models. The in vitro dissolution from the drug delivery systems (DDS) was explained by the Weibull model. The DDS efficacy was evaluated using two human colorectal carcinoma cell lines, HCT-15 and RKO. Unloaded zeolites presented no toxicity to both cancer cells, while all DDS allowed an important potentiation of the 5-FU effect on the cell viability. Immunofluorescence studies provided evidence for zeolite-cell internalization.